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Immunofluorescent staining of Human melanoma cells, utilizing Rabbit polyclonal to acetyl Lysine (ab21623) at 1:a hundred dilution. Note that the side chain has three methylene groups, so that regardless that the terminal amino group will be charged beneath physiological circumstances, the aspect chain does have important hydrophobic character. Incubate the membrane for 20 min after which wash with DMF to remove uncoupled amino acids.
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